Tissuedistribution study:· Plasma absorptionof paclitaxel was nearly negligible at 6 h In case of Taxol®, and it was readilyclean and uptake by the liver, spleen and lung. The plasma concentration was sustainedfor up to 24 h ,when paclitaxel was encapsulated in liposomes, · Ascompare to conventional liposomes, the plasma concentration level was greaterin PEGylated liposomes after conducting pharmacokinetic test in rats.· Durationof 6 and 24 h of pharmacokinetic study of the Taxol® in Tumor tissue, efficiency ofPEGylated liposomes show significantly higher than that of conventionalliposomes and also PEGylated paclitaxel liposome concentration was more intumor than in spleen, lung, heart, kidney and brain tissues from 6h. from theseresults we can judge the localization effect PEGylated liposomes in tumortissues · It looklike that slow discharge and long-circulating period of PEGylated liposomescould offer sufficient chance for paclitaxel to be achieved at the tumor sitethrough the EPR effect and preserve the effective therapeutic concentration fora long period of time through the depot effect. · Therefore,these results indicate that our PEGylated liposomal formulation purposelyincreased the antitumor effectiveness while weakening the potential alternativeor side-effects.
Inhibitionof tumor growth:· becausethe paclitaxel loaded classical liposomes and PEGylated liposomes have beenpretty stored inside the tumor tissues of MDA-MB-231 human breast most cancersxenograft model, the tumor growth inhibition impact was in additionanticipated. The examine at the manipulate (saline) group stopped at the thirtyfifth day motive is that the tumor capacity was extremely enlarged (about 2000mm3), while other agencies lasted till the sixtieth day. · The PEGylated liposomes inhibited tumor boom mostsuccessfully, accompanied by way of the traditional liposomes and Taxol® (p< zero.05).
This improved anti-tumor pastime of the PEGylated liposomes maybe clarified by means of the expanded nearby attention of pacltiaxel near thetumor thru EPR effect.